The Ufm1 Cascade

The Ufm1 Cascade

The ubiquitin-fold modifier 1 (Ufm1) is a posttranslational modifier that belongs to the ubiquitin-like protein (UBL) family. Ufm1 is present in nearly all eukaryotic organisms, with the exception of fungi. It resembles ubiquitin in its ability to be ligated to other proteins, as well as in the mechanism of ligation. While the Ufm1 cascade has been implicated in endoplasmic reticulum functions ...

The Ufm1-cascade of C. elegans

Background: The novel ubiquitin-like modifier (UBL) Ufm1 has essential functions in mammalian embryonic development. Results: The Ufm1-cascade in Caenorhabditis elegans is required for normal growth and development and is involved in cellular stress response. Conclusions: The C. elegans Ufm1-cascade is an antagonist of the unfolded protein response Significance: The C. elegans Ufm1-cascade offe...

Conformational locking of Ufm1 upon binding to the Ufm1-interacting sequence of Uba5

Deletion of Ubiquitin Fold Modifier Protein Ufm1 Processing Peptidase Ufsp in L. donovani Abolishes Ufm1 Processing and Alters Pathogenesis

Previously, we showed Leishmania donovani Ufm1 has a Gly residue conserved at the C-terminal region with a unique 17 amino acid residue extension that must be processed prior to conjugation to target proteins. In this report, we describe for the first time the isolation and characterization of the Leishmania Ufm1-specific protease Ufsp. Biochemical analysis of L. donovani Ufsp showed that this ...

UFMylation: A Unique & Fashionable Modification for Life

Ubiquitin-fold modifier 1 (UFM1) is one of the newly-identified ubiquitin-like proteins. Similar to ubiquitin, UFM1 is conjugated to its target proteins by a three-step enzymatic reaction. The UFM1-activating enzyme, ubiquitin-like modifier-activating enzyme 5 (UBA5), serves as the E1 to activate UFM1; UFM1-conjugating enzyme 1 (UFC1) acts as the E2 to transfer the activated UFM1 to the active ...